Purification and Regulatory Properties of Fructose 1,6-Diphosphatase from Hydrogenomonas eutropha
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چکیده
منابع مشابه
Yields of Hydrogenomonas eutropha from Growth
Molar growth yields were determined from chemostat cultures of Hydrogenomonas eutropha on succinate and on fumarate. The yields from culture on succinate were about 12 g higher than on fumarate. Assuming this difference to be equivalent to 1 molecule of adenosine triphosphate, it is concluded that the oxidation by oxygen of the Hydrogenomonas cytochrome b yields 1 molecule of adenosine triphosp...
متن کاملMixotrophic growth of Hydrogenomonas eutropha.
Mixotrophic growth conditions were established by the addition of lactate to cultures of Hydrogenomonas eutropha growing autotrophically in a gaseous environment of H(2), O(2), and CO(2) (6:2:1). The specific growth rate of mixotrophic cultures was double that of the autotrophic cultures, and lactate disappearance paralleled growth. Growth yields in mixotrophic cultures were significantly great...
متن کاملNutritional requirements for Hydrogenomonas eutropha.
Repaske, Roy (National Institute of Allergy and Infectious Diseases, Bethesda, Md.). Nutritional requirements for Hydrogenomonas eutropha. J. Bacteriol. 83: 418-422. 1962.-A simple apparatus for the autotrophic cultivation of Hydrogenomonas eutropha in 100-ml shake cultures is described. Nitrogen, in the form of ammonium, nitrate, or urea, was used for growth; nitrite could not be utilized. Opt...
متن کاملThe Electron Transport System of Hydrogenomonas eutropha
Wosilait and Nason (1) demonstrated that quinones could serve as electron acceptors for the enzymatic oxidation of reduced pyridine nucleotides, and their survey of plant, animal, and microorganism extracts (2) revealed the general occurrence of reduced pyridine nucleotide-quinone reductase activity. This finding and Dam’s (3) earlier report, which indicated that vitamin K was a common constitu...
متن کاملPARTIAL PURIFICATION AND PROPERTIES OF L-GLUTAMINE: D-FRUCTOSE 6-P AMIDOTRANSFERASE FROM HUMAN PLACENTA
The first enzyme of the pathway for uridine diphosphate N-acetyl-D-glucosamine (UDPAG) biosynthesis i.e. L-glutamine: D-fructose 6-P amidotransferase (E.C. 2.6.1.16) was purified 52-fold from human placenta using methanol fractionation and column chromatography on DEAE-Sephadex A-50. The enzyme showed optimal activity in a broad range of pH from 5.8 to 7.8 in both phosphate and cacodylate ...
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ژورنال
عنوان ژورنال: Journal of Bacteriology
سال: 1974
ISSN: 0021-9193,1098-5530
DOI: 10.1128/jb.120.1.304-310.1974